Dear Agnes, we have successfully labeled the Dicty nuclear envelope nicely with lamin NE81, Sun1, Nup53 and Nup62. All the best, Ralph Am 31.07.2013 um 10:59 schrieb "Miermont, Agnes" <[log in to unmask]>: > Dear all, > I would like to label the nuclear envelop of dicty. > I have first try using GFP-CAAX but it does not seem to work: I just obtain low cytoplasmic fluorescence. > I am now trying to label Nucleoporins, I have selected NUP62 as the yeast homolog, Nsp1 tagged with GFP present a nice labeling at the nuclear membrane. I would like to know if anybody has ever try labeling Nucleoporins or have any other idea for nuclear envelop labeling. > Thank you for your help, > Agnes > > > > ------------------------------------------------------------------------------------------------------------------------------- > Agnès Miermont, PhD > Department of Cell and Developmental Biology > MRC Laboratory for Molecular Cell Biology > University College London > Gower Street > London > WC1E 6BT > --------------------------------------------------------------------------------------------------------------------------- --------------------------------------------------------- Prof. Dr. Ralph Gräf Universität Potsdam Institut für Biochemie und Biologie ZELLBIOLOGIE Karl-Liebknecht-Strasse 24-25, Haus 26 14476 Potsdam-Golm Germany Tel.: +49-(0)331 9775520 FAX: +49-(0)331 9775522 http://www.bio.uni-potsdam.de/professuren/zellbiologie