On September 16th, there will be a live webinar presentation given by Dr. Kristi 
Hohenstein, to discuss a novel technique for stem cell propagation and EB generation 
using laser enabled analysis and processing technology.

 

During the webinar, Dr. Hohenstein will share results from a novel method for more 
efficient, standardized passage of embryonic stem cell cultures. The method can also be 
used for direct generation of embryoid bodies of specific sizes. The results from both 
experiments demonstrate two highly reproducible methods for propagation of large-scale 
stem cell cultures and can significantly improve the efficiency of ESC/iPSC differentiation 
for generation of specialized cell types to be used in cell-based screening and 
therapeutics.

 

Title: Standardizing Stem Cell Cultures to Maximize Differentiation Potential

Date:  Wednesday, September 16, 2009 

Time:  10:00 am – 11:00 am PST

 

Please reserve your Webinar seat at:
https://www2.gotomeeting.com/register/490910290 

 

After registering you will receive a confirmation email containing information about 
joining the Webinar.

 

Abstract
Derivation and propagation of embryonic and induced pluripotent stem cell (ESC/iPSC) 
lines are common tasks in stem cell biology, yet typical procedures vary extensively 
among laboratories. Manual methods are generally preferred because they result in 
consistent stem cell cultures, whereas enzymatic techniques are more widely used due to 
their scalability, but at the expense of reproducibility. To address these challenges, 
Cyntellect has developed innovative stem cell applications using the LEAPTM cell 
processing Workstation.  More specifically, the Stem Cell Passage application is a novel 
approach for efficient, standardized passage of embryonic stem cell cultures.  LEAP uses 
a laser to section undifferentiated stem cell colonies into cell clusters of defined size, 
resulting in more uniform colonies than current methodologies. A second novel 
application, EB Generation, provides the direct generation of embryoid bodies of specific 
sizes. This unique application is used to physically section stem cell cultures into defined 
sizes for the purpose of generating homogenous, uniformly-sized EB populations. Results 
demonstrate specific sized EBs differentiate more efficiently into neurons and 
cardiomyocytes than typical heterogeneous EB cultures.  These novel applications provide 
automated, reproducible methods for propagation of large-scale ESC/iPSC cultures and 
can significantly improve the efficiency of ESC/iPSC differentiation for generation of 
specialized cell types for cell-based screening and therapeutics.