Dear all,

since more than two months we observe very irregular growth of our  
wild type AX2:
the doubling time varies as it seems at random between 10 and more  
than 40 hours.

All the usual parameters including temperature, medium (we use  
Formedium HL5 medium with glucose), water, residual detergent in  
flasks from the dish washer, pH, light or culture density have been  
checked and they all do not seem to be the reason for this behavior.

My questions are the following:

Is this also observed in other labs?
What is the usual doubling time of AX2 in your hands?
How constant is that doubling time?
How many of you have tried to supplement the medium with vitamins, as  
stated in the technical comments on Dictybase, and how successful was  
that?

Your input is much appreciated,

Chris


Christian Hammann

Heisenberg research group ribogenetics

Faculty of Biology

Technical University Darmstadt

Schnittspahnstr. 10

64287 Darmstadt

t +49 6151 – 16 3955

f +49 6151 – 16 7440

e [log in to unmask]