Dear DictyBase Colleagues,

Does someone have an antibody that recognizes a phosphorylated Dicty  
protein or peptide during vegetative growth, but not its non- 
phosphorylated counterpart?

We are trying to test if a Vibrio cholerae effector is secreted into  
the Dicty cytosol. As an alternative to classical fractionation  
experiments - which are underway - we are thinking about tagging our  
effector with a peptide that is recognized by a Dictyostelium kinase.  
If we detect phosphorylated effector in infected amoebae, it would  
suggest that the bacterial protein localized to the host cytosol.

Thank you,
Stefan

Stefan Pukatzki, Ph.D.
Assistant Professor of Medical Microbiology & Immunology
University of Alberta
1-63 Medical Sciences Building
Edmonton, Alberta T6G 2H7







phone:  (780) 492-0904
fax:	       (315) 875-2031

http://www.ualberta.ca/~pukatzki/labpage/Welcome.html