In principel, Xin-Hua is right - but do not underestimate 
the number of regulatory RNAs, far-away promoter elements, 
etc. The EST map that we have is not very rich. When you 
select a landing region you may guess, but you do not know 
if you disrupt anything important!

Has anybody done it? (I mean selected a landing region and 
inserted something without any (!) phenotypic/biochemical 
effect?
wolfgang



On Wed, 9 Oct 2013 10:01:00 -0500
  Liao Xinhua <[log in to unmask]> wrote:
> Actually in our routine practice of gene disruption, we 
>insert genome with
> drug (e.g. blasticidin) resistant gene at the same time.
> 
> So principally, there is no difference between 
>disruption of target gene
> and insertion of gene into target region. The only thing 
>you need to
> consider is to choose a “landing region” for insertion. 
>Landing region
> should be intergenic region with dense genes around 
>(indicating active
> transcription), and has ubiquitous transcriptional 
>activity (reflected by
> broad spatial and temporal EST expression patterns).
> 
> Good luck,
> 
> Xin-Hua Liao
> 
> 
> 2013/10/8 John B. Biggins 
><[log in to unmask]>
> 
>> Has anyone had any success in targeted insertion of 
>>genes within the Dicty
>> genome?
>>
>> That is, you can stably insert the DNA you want in the 
>>region you choose?
>>
>> Thanks
>>
>> John

Wolfgang Nellen
Abt. Genetik
Univ. Kassel
Heinrich-Plett-Str. 40
34132 Kassel
Germany