dictyNews

Electronic Edition

Volume 46, number 3

January 24, 2020



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Abstracts

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Formins specify membrane patterns generated by propagating actin waves  



Mary Ecke1, Jana Prassler1, Patrick Tanribil1, Annette Müller-Taubenberger2, 

Sarah Körber3, Jan Faix3 and Günther Gerisch1 



1 Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 

Martinsried

2 LMU Munich, Department of Cell Biology (Anatomy III), Biomedical Center, 

D-82152 Planegg-Martinsried, Germany

3Hannover Medical School, Department of Biophysical Chemistry, 

Carl-Neuberg-Str.1, D-30625 Hannover, Germany





Molecular Biology of the Cell, accepted



Circular actin waves separate two distinct areas on the substrate-attached 

cell surface from each other: an external area from an inner territory that is 

circumscribed by the wave. These areas differ in composition of actin-

associated proteins and of phosphoinositides in the membrane. At the 

propagating wave, one area is converted into the other. By photo-conversion 

of Eos-actin and analysis of actin network structures we show that both in the 

inner territoriy and the external area the actin network is subject to continuous 

turnover. To address the question of whether areas in the wave pattern are 

specified by particular actin polymerizing machines, we locate five members 

of the formin family to specific regions of the wave landscape, using TIRF 

microscopy and constitutively active formin constructs tagged with fluorescent 

protein. Formin ForB favors the actin wave, ForG the inner territory, whereas 

ForA, ForE and ForH are more strongly recruited to the external area. 

Fluctuations of membrane binding peculiar to ForB indicate transient states 

in the specification of membrane domains prior to differentiation into ForB 

decorated and depleted ones. Annihilation of the patterns by 1 µM of the formin 

inhibitor SMIFH2 supports the implication of formins in their generation.





submitted by:  Mary Ecke [[log in to unmask]]

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[End dictyNews, volume 46, number 3]