People doing 31P NMR on live Dicty cultures have bubbled air through the cells in the NMR tube, I tried this and the cells seemed to be happy
Richard Gomer
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From: DICTY [[log in to unmask]] on behalf of Jon Moore [[log in to unmask]]
Sent: Saturday, February 20, 2016 10:21 AM
To: [log in to unmask]
Subject: [DICTY] Anyone ever bubble instead of shake?
Hi.
There is a bit of a long and uninteresting story behind this one,
but has anyone ever bubbled their dicty cultures for agitation
instead of shaking? If so, were there any complications?
Peace,
Jon Moore
Assistant Professor of Biology
& Lab Coordinator
Pomona College
Claremont, California 91711