Be aware that switching to a different food source will have dramatic 
consequences in gene expression and epigenetics. These may not be 
obvious on the first glance. There is a recent report on S. pombe 
where a specific tRNA methylation changed from zero to 100% by rather 
small changes in the medium.


On Fri, 19 Apr 2013 21:08:38 +0000
  Joan Strassmann <[log in to unmask]> wrote:
> Dear Everyone,
> 
> We have a strain of E. coli that Dicty just love to eat.  It is 
>originally from Richard Gomer's lab where somewhere along the line 
>either in our lab or his, KA got replaced by E coli.  The point is 
>Dicty love to eat it.  It is not classified as pathogenic. I don't 
>know if we've sequenced it.
> 
> We are trying to switch to using it in everything.  We had to become 
>a BL2 lab instead of BL1 (USA thing) for other reasons anyway, but 
>still prefer this.
> 
> We could send this to the stock center.
> 
> Cheers,
> 
> Joan
> 
> On Apr 19, 2013, at 3:56 PM, Knecht, David wrote:
> 
> But as Robert indicated, it would be nice to have a strain that is 
>verified as NOT pneumoniae that we could get from Dictybase stock 
>center so we don't have to worry about potential health issues when 
>using it in classes and the lab.  Dave
> 
> On Apr 19, 2013, at 3:06 PM, Richard Sucgang PhD 
><[log in to unmask]<mailto:[log in to unmask]>>
> wrote:
> 
> I forgot to mention that unlike the different Dicty lineages, we are 
>less careful about how we bank the feeder bacteria we use. For 
>example, what one lab calls Ka may be significantly deviated from a 
>different lab's, perhaps even exhibiting slightly different 
>serotypes, but as long as they grow at predictable rates in the 
>media, and Dicty will eat them, it really doesn't impact what we do.
> 
> That said, what sequences we retrieved from the contaminating 
>bacteria matched the Kp genome well enough to identify them as such. 
>Something to consider as a genome project is executed.
> 
> On Apr 19, 2013, at 1:22 PM, Pierre Cosson 
><[log in to unmask]<mailto:[log in to unmask]>> wrote:
> 
> Dear all,
> Has the genome of the dicty Klebsiella (Ka) strain ever been 
>sequenced? And does anybody have information about this bug (e.g. 
>serotype)? If not we may be willing to give it a try.
> Best
> Pierre Cosson
> 
> 
> 
> Pierre Cosson
> Centre Medical Universitaire
> Dpt of Cell Physiology and Metabolism
> 1 rue Michel Servet
> CH1211 Geneva 4, Switzerland
> 
> Email:[log in to unmask]<http://unige.ch>
> Tel. (41) 22 379 5293
>Fax (41) 22 379 5338
> 
> <PastedGraphic-1.tiff>
> 
> David Knecht, Ph.D.
> Professor and Head of Microscopy Facility
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
> 
> _________________________________
> Joan E. Strassmann
> Professor of Biology
> 
> Department of Biology
> Washington University in St. Louis
> One Brookings Drive
> Campus Box 1137
> St. Louis MO 63130
> 
> phone: (314) 935-3527  fax: (314) 935-4432
> cell: (832) 978-5961
> skype: strassm
> e-mail [log in to unmask]<mailto:[log in to unmask]>
> http://strassmannandquellerlab.wordpress.com/
> 
> Blogs: http://sociobiology.wordpress.com/
> http://slowbirding.wordpress.com/
> http://goodbyehouston.wordpress.com/
> Twitter: @JoanStrassmann
> 
> 
> 
> 
> 

Wolfgang Nellen
Abt. Genetik
Univ. Kassel
Heinrich-Plett-Str. 40
34132 Kassel
Germany