mRaspberry works great. It's an RFPmars recombinant fluoro protein. I 
like it because it's in the far red spectra like Texas Red and minimizes 
green autofluorescence better than Lo-flo and thus less bleed-through. 
Clontech offers it. But it is pricey.

Just to add to Douwe's comments, I too have experienced unacceptable 
fluorescence or absolutely no fluorescence from mRFPmars to the point 
that we simply do not use that vector. More often than not it fails to 
produce a signal even though the message and the fusion protein are 
detectable by both RT-PCR and Western.

Mike

On 2013-07-01 18:40, Chubb, Jonathan wrote:
> Hi Douwe, Annette,
> mKate and Td Tomato apparently work well as RFPs.
> Have either of you (or anyone else) tried these in Dicty?
> Thanks,
> Jon
>
>
>
>
>
> On 01/07/2013 22:55, "Douwe Veltman" <[log in to unmask]> 
> wrote:
>
>>I find that GFP fusions generally work better than RFP fusions. RFP 
>> is
>>almost always less bright and sometimes simply fails to express.
>>
>>I use both mRFPmars and codon-optimized mCherry and they behave very
>>similarly. The only advantage of mCherry it that it is not excited 
>> with
>>the 488 laser, which is used to excite GFP. This can prevent unwanted
>>bleed-through and bleaching.
>>
>>TagRFP is a different protein. Sometimes a fusion that does not work 
>> with
>>mRFPmars/mCherry (no expression, aggregation), works well with 
>> TagRFP. The
>>disadvantage of TagRFP is that it bleaches very rapidly. The 
>> photostable
>>version of TagRFP circumvents this, but in my hands this protein was 
>> not
>>very bright.
>>
>>Douwe Veltman
>>
>>
>>
>>> Hello all,
>>>
>>> Could anyone please share the experience of expressing mCherry 
>>> fusion
>>> proteins in Dictyostelium? Does mCherry express well in Dicty?
>>>
>>> Thank you.
>>>
>>> Weiye
>>>
>>> Weiye Wang
>>> Laboratory of Cellular and Molecular Biology
>>> National Cancer Institute
>>> Bldg 37, Rm 2056
>>> 37 Convent Drive
>>> Bethesda MD 20892
>>> Ph# 301-594-0768
>>>
>>

-- 
Sent from a nanochip implanted in my brain.
-------------------------------------------------
Michael A. Myre, PhD
Instructor of Neurology, Asst. in Genetics
Center for Human Genetic Research
Massachusetts General Hospital
Harvard Medical School
185 Cambridge St. Rm. 5.612A
Boston, MA 02114
Ph. 617-643-5536
[log in to unmask]
Website: http://chgr.org/index-faculty_myre.html
Website: 
http://www.massgeneral.org/neurology/researcher_profiles/myre_michael.aspx
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