Hi Douwe, Annette,
mKate and Td Tomato apparently work well as RFPs.
Have either of you (or anyone else) tried these in Dicty?
Thanks,
Jon





On 01/07/2013 22:55, "Douwe Veltman" <[log in to unmask]> wrote:

>I find that GFP fusions generally work better than RFP fusions. RFP is
>almost always less bright and sometimes simply fails to express.
>
>I use both mRFPmars and codon-optimized mCherry and they behave very
>similarly. The only advantage of mCherry it that it is not excited with
>the 488 laser, which is used to excite GFP. This can prevent unwanted
>bleed-through and bleaching.
>
>TagRFP is a different protein. Sometimes a fusion that does not work with
>mRFPmars/mCherry (no expression, aggregation), works well with TagRFP. The
>disadvantage of TagRFP is that it bleaches very rapidly. The photostable
>version of TagRFP circumvents this, but in my hands this protein was not
>very bright.
>
>Douwe Veltman
>
>
>
>> Hello all,
>>
>> Could anyone please share the experience of expressing mCherry fusion
>> proteins in Dictyostelium? Does mCherry express well in Dicty?
>>
>> Thank you.
>>
>> Weiye
>>
>> Weiye Wang
>> Laboratory of Cellular and Molecular Biology
>> National Cancer Institute
>> Bldg 37, Rm 2056
>> 37 Convent Drive
>> Bethesda MD 20892
>> Ph# 301-594-0768
>>
>