O-

Pls check our EMBO J contractile vacuole paper a few yrs ago. 

Best-

R

Richard A. Firtel
Professor
Associate Dean for Operations
Division of Biological Sciences
UCSD
La Jolla, CA 92093-0380
Tel +1-858-534-2788
fax +1-858-822-5900

On Oct 4, 2010, at 7:50 PM, "Oliver Hoeller" <[log in to unmask]> wrote:

>  Dear Dicty workers,
> please let me pick your brains for the following:
> I'd like to redirect cytoplasmic proteins to new places within the cell 
> upon a stimulus ( e.g. using a small molecule as a hetero-dimerization 
> agent). This could be achieved by having a cytoplasmic recruitee bind to 
> a recruiter on an organelle (ER, Golgi, Mitochondria...).
> For that to work I'd like help with finding suitable recruiters. In 
> other words -  markers for organelles that you or others have used 
> successfully in vivo. These proteins should also contain a cytoplasmic 
> portion that can tolerate fusion to be useful.
> 
> So far I have successfully used calnexin, which however failed later for 
> other reasons.
> 
> All hints are appreciated!
> 
> Thanks a lot,
> -Oliver
> 
> 
> -- 
> 
> o l i v e r   h o e l l e r
> postdoc
> lim&  weiner labs
> ucsf