Dear Ted,
I am recently working with a protein that has a single HA tag at its C-terminus.
We are using the roche monoclonal rat Ab 3F10, that Manu Dubin mentioned.
It works very well in western blot (1: 2000) as well as in IFs (1: 100-200).
Best
Tanja


-------- Original-Nachricht --------
> Datum: Thu, 19 Aug 2010 12:28:56 -0400
> Von: Ted Cox <[log in to unmask]>
> An: [log in to unmask]
> Betreff: [DICTY - 390] HA tags

> Dear All,
>
> We've had trouble detecting a single C-terminal HA tag in transformed
> cells, both in cell extracts, and to a lesser extent, with partially
> purified proteins.  We think it may be the primary antibody--there are
> many sources.  Any observations and help would be greatly appreciated.
>
> Ted
>
> Edward Cox
> Professor of Molecular Biology
> Edwin Grant Conklin Professor of Biology
> 333 Moffett Lab
> Phone: 609-258-3856
> Lab Phone: 609-258-3571
> Fax: 609 258 6730
>
> [log in to unmask]
> http://www.molbio2.princeton.edu/index.php?option=content&task=view&id=206

Tanja Riyahi
Institut für Biochemie I
Medizinische Fakultät
Universität zu Köln
Joseph-Stelzmann-Str. 52
50931 Koeln
Tel.: 0221-478-6988
FAX:  0221-478-6979