Hi Sascha,

I've used FM1-43 (at 5uM) and CM DeepRed (at 0.5ug/ml) in FACS analysis
before which have worked extremely well. Just add the dye for the desired
time, wash once in ice cold buffer and they're ready to go!

Tom

> Dear Dicty-researchers,
>
> First of all I would like to wish everybody a happy and successful new
> year
> 2016.
>
> And here is now my question: has anybody tried to label the cell
> surface/membrane of Dicty amoebae for FACS-analysis? Any protocol or
> advice
> will be helpful.
> Thanks a lot and kind regards,
>
> Sascha.
> --
> Dr. Sascha Thewes
> Institut für Biologie - Mikrobiologie
> Fachbereich Biologie, Chemie, Pharmazie
> Freie Universität Berlin
> Königin-Luise-Str. 12-16
> D-14195 Berlin
>
> Tel.: 030-838-53373
> Email: [log in to unmask]
> Web:
> http://www.biologie.fu-berlin.de/arbeitsgruppen/mikrobiologie/ag_mutzel/inde
> x.html
>
>
>