Wanessa,
I have used two different kits for RNA extraction and both the kits give high yields of total RNA.
1. SV Total RNA Isolation System from Promega Inc.
2. AurumTM Total RNA Mini Kit (Bio-Rad Laboratories Inc., Hercules, CA).
Best,
Sandhya.

 Dr. Sandhya N Baviskar
Assistant Professor of Cell Biology
Department of Biological Sciences
University of Arkansas- Fort Smith
5210 Grand Ave.
Fort Smith AR 72913
Phone : 479-788-7789
Fax: 479-424-6789
________________________________________
From: DICTY [[log in to unmask]] On Behalf Of Wanessa Cristina DE LIMA [[log in to unmask]]
Sent: Wednesday, January 25, 2012 3:41 AM
To: [log in to unmask]
Subject: [DICTY - 737] Comercial kits for RNA extraction

 Dear all,

Does anyone have suggestions for good commercially available kits for RNA extraction? I'm using a NucleoSpin kit from Macherey-Nagel, but yields (at least in my hands) are normally bad (i  use 5-10 million cells/prep).

Thanks a lot for the help,
Wanessa de Lima
(lab Pierre Cosson, UNIGE, Geneva)