Hi Kari,

we've developed a system for non-ligation cloning of knock out vectors  
for Dicty together with the company IBA in Goettingen, Germany.
The reference of that system is PMID: 21316445 and the vector pKOSG- 
IBA-Dicty1 can be found at http://www.iba-go.com/naps/naps_sg_av_new.html 
.

Best regards,
Christian

On Nov 10, 2011, at 4:40 PM, Kari Naylor wrote:

> Dear Dicty colleagues
>
> I am writing to see if anyone can help us on two disparate issues.
>
> 1) DAPI staining.  We'd love some success stories on DAPI staining.   
> We've fixed the cells as recommended (in 3% glutaldehyde- maybe this  
> is the problem) but the staining is quite poor. Any suggestions  
> would be appreciated.
>
>
> 2) we are trying to make a knockout strain and are having some  
> significant issues attaching our up and down stream genomic pieces  
> to BSR gene.  We have decided to try pricing out some companies that  
> synthesis large pieces of DNA, but to do that we need the entire  
> sequence of the BSR gene (we're using pUCBsrdeltaBam).  Does anyone  
> have the complete sequence???
>
>
> Thanks in advance
>
> Kari
>
> Kari Naylor PhD
> University of Central Arkansas
> Biology Department
> 139 Lewis Science Center
> ph:501-450-5826
> [log in to unmask]


________________________________

Christian Hammann

Heisenberg research group ribogenetics

Faculty of Biology

Technical University Darmstadt

Schnittspahnstr. 10

64287 Darmstadt

t +49 6151 – 16 3955

f +49 6151 – 16 7440

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