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April 2013, Week 3

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Richard Sucgang PhD <[log in to unmask]>
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Fri, 19 Apr 2013 14:06:01 -0500
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I forgot to mention that unlike the different Dicty lineages, we are less careful about how we bank the feeder bacteria we use. For example, what one lab calls Ka may be significantly deviated from a different lab's, perhaps even exhibiting slightly different serotypes, but as long as they grow at predictable rates in the media, and Dicty will eat them, it really doesn't impact what we do. 

That said, what sequences we retrieved from the contaminating bacteria matched the Kp genome well enough to identify them as such. Something to consider as a genome project is executed.

On Apr 19, 2013, at 1:22 PM, Pierre Cosson <[log in to unmask]> wrote:

> Dear all,
> Has the genome of the dicty Klebsiella (Ka) strain ever been sequenced? And does anybody have information about this bug (e.g. serotype)? If not we may be willing to give it a try. 
> Best
> Pierre Cosson
> 
> 
> 
> Pierre Cosson
> Centre Medical Universitaire
> Dpt of Cell Physiology and Metabolism
> 1 rue Michel Servet
> CH1211 Geneva 4, Switzerland
> 
> Email:[log in to unmask]
> Tel. (41) 22 379 5293
> Fax (41) 22 379 5338
> 
> <PastedGraphic-1.tiff>

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