We evaluated it to approx 30micro-molar in our HL5, but since it all  
comes from contaminants, it may vary significantly from one lab to  
another, even using the same recipe.
Pierre


On 18 janv. 12, at 20:22, Steve Charette wrote:

> Dear Dicty colleagues,
>
> I have a simple question for you...
>
> What is the concentration of calcium in HL5 medium?
>
> Thank you in advance for the answer and the reference if possible.
>
> Steve
> ----------------------------------------------------------------------------
> ----------------------------------------------------------------------------
> Steve Charette, Ph. D.
> Professeur adjoint (Dépt. Biochimie, microbiologie et bio- 
> informatique,
> Université Laval)
>
> [log in to unmask]
>
> WEBSITE: www.amibe.org
>
> PRIMARY AFFILIATION
> Institut de Biologie Intégrative et des Systèmes (IBIS)
> Pavillon Charles-Eugène-Marchand
> 1030, avenue de la Médecine
> Université Laval
> Québec, Qc
> G1V 0A6, CANADA
> Office: 418 656-2131 ext. 6914
> Fax:    418 656-7176
>
> SECONDARY AFFILIATION
> Centre de recherche,
> Inst. Uni. de cardiologie et de pneumologie de Québec
> 2725 Chemin Sainte-Foy
> Québec, Qc
> G1V 4G5, CANADA
> Office: 418 656-8711 ext. 3311

Pierre Cosson
Centre Medical Universitaire
Dpt of Cell Physiology and Metabolism
1 rue Michel Servet
CH1211 Geneva 4, Switzerland
Email:[log in to unmask]
Tel. (41) 22 379 5293
Fax (41) 22 379 5338