Hi-
We did, many years ago, in this paper

A protein containing a serine-rich domain with vesicle fusing properties mediates cell cycle-dependent cytosolic pH regulation.
Brazill DT, Caprette DR, Myler HA, Hatton RD, Ammann RR, Lindsey DF, Brock DA, Gomer RH.
J Biol Chem. 2000 Jun 23;275(25):19231-40.

we followed cell lineages over many hours by videomicroscopy, then we gently changed to medium to medium containing the dye BCECF-AM, then imaged at high power individual cells in the field using two different fluorescence filter cubes - it was difficult....
there may be better dyes nowdays

cheers

Richard Gomer
Department of Biology
Texas A&M University
301 Old Main Drive
College Station, TX  77843-3474
979 458 5745
________________________________________
From: DICTY [[log in to unmask]] on behalf of Marianne Grafe [[log in to unmask]]
Sent: Tuesday, October 8, 2019 9:20 AM
To: [log in to unmask]
Subject: [DICTY] pH-sensitive dyes

Dear All,

Has anyone worked with pH-sensitive dyes to detect the cytosolic pH in
Dictyostelium?

Thanks,
Marianne

--
Marianne Grafe
PhD student
Group Cell Biology
Institute of Biochemistry and Biology
University of Potsdam
Karl-Liebknecht-Straße 24, Haus 26
14476 Potsdam
Germany
Tel.: +49-(0)331 977-5555