Dear Ralph,
Marin and Rothman (1980, JCB 87, 823) give ionic contents of A3
vegetative cells measured by flame photometry (Na 8.2, K 46.5 meq/L).
Maeda (1983, Bot Mag 96, 193) reports ionic contents of D. d. NC-4
vegetative cells by atomic absorption spectroscopy (35.0, 3.6, 10.6,
and 2.3 for K, Na, Mg, and Ca, respectively). She further
demonstrates the effects of external Na and K concentrations. I
measured Na, K and Cl of NC-4 and D. mucoroides veg cells by flame
photometry and chloridometry (unpublished) and obtained Na and K
values comparable to Maeda's values. The Cl concentration was always
about twice that of Na.
Cytosolic pH is most likely between 7 and 7.5. See appendix of Inouye
(1988, JCS 91, 109).
Maeda (1983)
http://springerlink.com/content/t106081564836261/fulltext.pdf
Inouye (1988)
http://jcs.biologists.org/cgi/reprint/91/1/109
These papers somehow cannot be found on Pubmed.
Best wishes,
Kei
_____________________________
Kei Inouye
Department of Botany
Graduate School of Science
Kyoto University
Sakyo-ku, Kyoto 606-8502
Japan
_____________________________
[log in to unmask]
http://cosmos.bot.kyoto-u.ac.jp/csm/
_____________________________
On 2010/06/24, at 20:54, Ralph Gräf wrote:
> Dear Dicty Researchers,
>
> I was trying to find a publication where somebody has measured or
> estimated the cytosolic salt concentrations of prominent cations
> and anions but I was not successful. I doubt that the commonly used
> low ionic strength Soerenson phosphate buffer reflects a
> physiological situation for cytosolic proteins. We would like to
> use an optimal physiological buffer for cytosolic proteins with
> regard to pH, ion concentration and ion composition. Any helpful
> hints are welcome.
>
> All the best,
>
> Ralph
>
>
> ---------------------------------------------------------
>
> Prof. Dr. Ralph Gräf
>
> University of Potsdam
> Institute of Biochemistry and Biology
> Department of CELL BIOLOGY
>
> Karl-Liebknecht-Strasse 24-25, Haus 26
> 14476 Potsdam
> Germany
>
> Tel.: +49-(0)331 9775520
> FAX: +49-(0)331 9775522
>
> http://www.bio.uni-potsdam.de/professuren/zellbiologie/aktivitaten
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