Hi Rob,

we also use MG132 in cell lysates. We use it at 
100µM but definitely could go to lower conc.
See Arhzaouy et al., PLos ONE, 2012.

Best wishes

Ludwig



At 18:53 05.03.2018, Richard Gomer wrote:
>Hi Rob-
>We (i.e. Patrick Suess) have used MG132, works 
>at 6 micromolar (and probably lower) on lysates, 
>12 micromolar to affect whole cells
>Lactacystin had no effect on whole cells.
>see Patrick's J. Cell Science 2017 paper for details
>cheers
>Richard
>
>Richard Gomer
>Department of Biology
>Texas A&M University
>301 Old Main Drive
>College Station, TX  77843-3474
>979 458 5745
>________________________________________
>From: DICTY [[log in to unmask]] 
>on behalf of Rob Kay [[log in to unmask]]
>Sent: Monday, March 5, 2018 11:38 AM
>To: [log in to unmask]
>Subject: [DICTY] Proteasome inhibitors
>
>Does anybody have recent experience of proteosome inhibitors with Dicty?
>Which ones work (and at what concentration) and which do not?
>
>This question was last asked in 2003, but 
>perhaps there has been progress since then.
>
>Thank you friends and colleagues for any help!
>
>Rob Kay
>
>
>
>Robert Kay,
>
>MRC Laboratory of Molecular Biology,
>
>Francis Crick Avenue,
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>Cambridge Biomedical Campus,
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>Phone, Office:+ 1223 267039 (01223 267039 from UK)
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