dictyNews
Electronic Edition
Volume 45, number 10
March 22, 2019
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Abstracts
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Transition state dynamics during a stochastic fate choice
Vlatka Antolovic, Tchern Lenn, Agnes Miermont,
Jonathan R. Chubb
Development, in press
http://dev.biologists.org/content/early/2019/03/18/dev.173740
The generation of multiple fates from a uniform cell population
via self-organisation is a recurring feature in development and
regeneration. However, for most self-organising systems, we
have little understanding of the processes that allow cells to
become different. One of the clearest examples of developmental
self-organisation is shown by Dictyostelium, with cells segregating
into two major fates, stalk and spore, within multicellular aggregates.
To characterise the gene expression decisions underlying this cell
fate bifurcation, we carried out single cell transcriptomics on
Dictyostelium aggregates. Our data show the transition of
progenitors into prespore and prestalk cells occurs via distinct
developmental intermediates. Few cells were captured switching
between states, with minimal overlap in fate marker expression
between cell types, suggesting states are discrete and transitions
rapid. Surprisingly, fate-specific transcript dynamics were a small
proportion of overall gene expression changes, with transcript
divergence coinciding precisely with large scale remodelling of
the transcriptome shared by prestalk and prespore cells. These
observations suggest the stepwise separation of cell identity is
temporally coupled to global expression transitions common to
both fates.
submitted by: Jonathan Chubb [[log in to unmask]]
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The AJ154 antibody recognizes the Dictyostelium p80 protein by Western blot
Wanessa Cristina Lima, Pierre Cosson
Antibody Reports, 2019, vol. 2, e23
https://oap.unige.ch/journals/abrep/article/view/23
The AJ154 antibody, derived from the H161 hybridoma, detects by Western
blot the full-length p80 protein from Dictyostelium discoideum.
submitted by: Wanessa du Fresne von Hohenesche [[log in to unmask]]
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The AJ154 antibody recognizes the Dictyostelium p80 protein by immunofluorescence
Wanessa Cristina Lima, Pierre Cosson
Antibody Reports, 2019, vol. 2, e24
https://oap.unige.ch/journals/abrep/article/view/24
The AJ154 antibody, derived from the H161 hybridoma, detects by immunofluorescence
the full-length p80 protein from Dictyostelium discoideum.
submitted by: Wanessa du Fresne von Hohenesche [[log in to unmask]]
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[End dictyNews, volume 45, number 10]
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