Hi Pascale-
I don't know about Dicty, but for human cells, we can get very good detachment and viability with Accutase (best price seems to be from Biolegend) in situations where trypsin EDTA doesn't work well.
I googled Accutase to check the spelling and the first item on the page is a free sample....

http://www.google.com/search?client=safari&rls=en&q=accutase&ie=UTF-8&oe=UTF-8

cheers

Richard Gomer
Professor of Biology
Texas A&M University
ILSB    MS 3474
301 Old Main Drive
College Station, TX  77843-3474
979 458 5745

________________________________________
From: DICTY [[log in to unmask]] on behalf of Charest, Pascale G - (pcharest) [[log in to unmask]]
Sent: Thursday, March 20, 2014 12:42 PM
To: [log in to unmask]
Subject: [DICTY] help detaching cells

Dear all,

We've been working with pretty adherent cells and it's becoming a pain to resuspend them when dealing with a lot of plates. Growth in suspension is not an option. I was wondering if anyone ever used a "detaching aid" such as trypsin or EDTA, which are used for mammalian cells. I'm especially wondering if the cells are OK for experiments after such treatments.

Thanks in advance!

Pascale


Pascale G Charest, PhD
Assistant Professor
Department of Chemistry and Biochemistry
University of Arizona
1041 E. Lowell Street
Bio Sciences West, Room 345
Tucson, AZ 85721-0088
Tel Office: 520-626-2916
Tel Lab: 520-621-9926