quite a few years back - I (with rick firtel, bill loomis, elliot 
rosen) looked at the heat shock response. i am pretty sure we saw 
heat shock at 30oC, with a significant unloading (~80 %?) in <30 min 
of polysomes in growing cells - in HL5 media. it is unlikely we tried 
Paul's trick, but from data in other systems, i would not expect that 
the heat shock response in growth to be protected by mannitol - 
certainly worth a try, though.

how would i reconcile paul's observation? polysomes in starved cells 
are already unloaded to 90%, with a preferential "loading" of 
develop. mRNAs. so mannitol may protect more generalized stress in development.

i am not sure any of this was ever published and bill and rick 
probably have more data/ideas.

alan kimmel



At 06:49 AM 10/5/2009, Gareth Bloomfield wrote:
>Hi,
>
>Regarding other Dicty species, it seems from the literature that even
>isolates from the tropics often struggle to grow and/or develop at 30
>degrees (eg a paper from Cavender -
>http://mic.sgmjournals.org/cgi/content/abstract/62/1/113). Leafing through
>Raper's 'The Dictystelids' candidates might be D. polycephalum (optimum
>temperature for fruiting 28-30 C), D. coeruleo-stipes (fruits between 15
>and 30), and D. rhizopdium (fruits at 30).
>
>If anyone had the opposite problem and needed an even less heat-tolerant
>species I could recommend D. septentrionalis, which is really beautiful
>and suffers anywhere above 20 degrees.
>
>Has anyone looked specifically for heat-tolerant isolates of D. discoideum?
>
>Best regards,
>
>Gareth
>
>
> > Hello,
> > Does anyone know of any relatively temperature tolerant strain that is
> > OK at 30 C or even a tad higher?  Or something similar to dicty?
> > I would dearly love to acquire it, if it exists.
> > Thanks,
> > Joy
> >