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July 2009, Week 3

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Subject:
Re: Mitochondrial potentiometric dyes
From:
Malgorzata Czarna <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Wed, 15 Jul 2009 23:29:18 +0200
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text/plain
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Hello Michelle,

In our work we have used MitoTracker Orange CMTMRos (Molecular Probes) at
the concentration 1 µM to study changes of mitochondrial membrane
potential in wild type cells.

Cheers,


Dr. Malgorzata Czarna
Institut für Biologie - Mikrobiologie
Fachbereich Biologie, Chemie, Pharmazie
Freie Universität Berlin
Königin-Luise-Str. 12-16
14195 Berlin

Tel.: 0049 30-838-53373
Fax: 0049 30-838-57773
Email: [log in to unmask]
Web: http://www.biocircle.fu-berlin.de/mikrobio2/index.php?id=mb2&p=9




> Dear All,
>
> Does anyone have any experience or advice regarding mitochondrial
> potentiometric dyes in Dictyostelium?
>
> I have tried out TMRM (tetramethylrhodamine methyl ester perchlorate) from
> Sigma at a variety of concentrations and incubation times, with little
> success. Has anyone managed to use this dye or perhaps a similar one?
>
> Thanks,
>
> Michelle Stevense
> Chubb Lab
> Dundee
> UK
>
> [log in to unmask]
> The University of Dundee is a registered Scottish charity, No: SC015096
>

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