Hi Mike,

no idea whether someone has already tried, but I would expect problems. Dicty introns are comparatively very small and the consensus sequences for recognition and splicing are a bit different (see attached).

Regards,
F. Rivero

-----Ursprüngliche Nachricht-----
Von: DICTY im Auftrag von Michael Koonce
Gesendet: Fr 08.04.2011 18:02
An: [log in to unmask]
Betreff: [DICTY - 533] Intron splicing
 
Hi Folks, 

Are Dicty introns efficiently spliced in mammalian cells? We have a genomic
clone of a kinesin we want to express in HeLa cells, and it contains a small
intron just upstream of a targeting region...

Thanks
Mike Koonce



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