Dear Alex,

It isn't clear which wild type-mutant mix you have carried out. We have
seen a preference on the part of wild type cells  to occupy the collar
region in mixes involving the wild type and the trishanku mutant (see
Jaiswal et al., Differentiation, Volume 74 Issue 9-10, Pages 596 - 607,
2006, "Trishanku, a novel regulator of cell-type stability and
morphogenesis in Dictyostelium discoideum").

As for wýld type ecmA-lacZ transformants by themselves, do you mean that
the tip does not show lacZ expression but the collar region does?

cheers

Vidya


> Hello everyone,
>
> I have been doing alot of studies in chimera, mainly by following
individual cell types using lacZ reporters.  One of the defects I see is
very strange - ecmA:lacZ (construct S) in the ecmO collar region. Even
more strangely is that in a mutant/wild type ecmA:lacZ mix, it is the
wild type cells that go there!
>
> Has anyone ever seen anything like it with things they are working on or
know of any papers where a similar phenotype has been seen?  Its a
difficult search term and i'm not having much luck.
>
> Cheers
> Alex
>





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