Hi　Sascha,

My impression is same as Pauline. Osmotic pressure of fixative may be low, 
and/or dehydration and emmbedding (substitution) is not suffisient.

M. Sameshima

----- Original Message ----- 
From: "Sascha Thewes" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Thursday, June 13, 2013 12:12 AM
Subject: [DICTY] EM mitochondria


Dear Dicty researchers,

We did in our lab EM pictures of Dicty cells and found some strange looking
mitochondria. Mitos seem to have wholes or inclusions (see arrows in the
attached image). As it is the first time for us to make EM pictures we are
not sure if we just see preparation artefacts or something else. Has anybody
suggestions?

Thanks for your help,

Sascha.

-- 
Sascha Thewes, PhD
Institute for Biology - Microbiology
Department of Biology, Chemistry, Pharmacy
Freie Universität Berlin
Königin-Luise-Str. 12-16
14195 Berlin
Germany

Phone: +49-(0)30-838-53373
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