Dear Stefan and Dictyologists,

Actually we are real fans of the BTX's ECM 830 square porator.  
Together with the attached ptotocole for an improved electroporation  
medium that ensures a better cell survival and hence use of more  
drastic poration conditions, we have excellent results.
Cheers,

Thierry


Quoting Stefan Pukatzki <[log in to unmask]>:

>
> Hi Everyone,
>
> We are in the market for a new electroporator, and are wondering if
> anyone out there is extremely happy with their instrument to transform
> amoebae. The choices are limited (Bio-Rad Gene Pulser Xcell Total
> system, The Eppendorf Multiporator, BTX's ECM 830, or Thermo
> Scientific's Celljec T Pro), but if anyone has any recommendations or
> reservations, we would like to hear about it.
>
> Thank you,
> Stefan
>
> Stefan Pukatzki, Ph.D.
> Assistant Professor of Medical Microbiology & Immunology
> University of Alberta
> 1-63 Medical Sciences Building
> Edmonton, Alberta T6G 2H7