Hi,
We have had those types of problems, but for a very simple reason. The motor
in our shaker platform was wearing out and would switch on and off.  You
might also want to check whether building temperature remains constant...

Cheers,
Mike



> Dear all,
> 
> since more than two months we observe very irregular growth of our
> wild type AX2:
> the doubling time varies as it seems at random between 10 and more
> than 40 hours.
> 
> All the usual parameters including temperature, medium (we use
> Formedium HL5 medium with glucose), water, residual detergent in
> flasks from the dish washer, pH, light or culture density have been
> checked and they all do not seem to be the reason for this behavior.
> 
> My questions are the following:
> 
> Is this also observed in other labs?
> What is the usual doubling time of AX2 in your hands?
> How constant is that doubling time?
> How many of you have tried to supplement the medium with vitamins, as
> stated in the technical comments on Dictybase, and how successful was
> that?
> 
> Your input is much appreciated,
> 
> Chris
> 
> 
> Christian Hammann



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