Hi Sascha,

I remember that Yasuo Maeda in one of his works ("Formation of a prespore
specific structure from a mitochondrion during development of the cellular
slime mold, Dictyostelium discoideum", Dev Growth Differ 13, 1971) showed that
in differentiating prespore cells some mitochondria can be transformed into
mitochondrion-vacuole complexes (or probably called PSV complexes). 
In this paper there are quite many photos included and, depending on your
experimental conditions, maybe you find something similar to your mitochondria.
I hope it can help,
Cheers,

Gosia Czarna 




Malgorzata Czarna, PhD,
University of Wroclaw
Faculty of Biotechnology
Molecular Biology of the Cell Dept
Przybyszewskiego 63/77
51-148 Wroclaw
Poland

Tel. 0048 71 375 62 73
Fax. 0048 71 375 62 34


On Wed, 12 Jun 2013 17:12:07 +0200, Sascha Thewes wrote
> Dear Dicty researchers,
> 
> We did in our lab EM pictures of Dicty cells and found some strange looking
> mitochondria. Mitos seem to have wholes or inclusions (see arrows in 
> the attached image). As it is the first time for us to make EM 
> pictures we are not sure if we just see preparation artefacts or 
> something else. Has anybody suggestions?
> 
> Thanks for your help,
> 
> Sascha.
> 
> -- 
> Sascha Thewes, PhD
> Institute for Biology - Microbiology
> Department of Biology, Chemistry, Pharmacy
> Freie Universität Berlin
> Königin-Luise-Str. 12-16
> 14195 Berlin
> Germany
> 
> Phone: +49-(0)30-838-53373
> Fax: +49-(0)30-838-57773
> Email: [log in to unmask]
> Web: 
> http://www.biologie.fu-berlin.de/arbeitsgruppen/mikrobiologie/ag_mutzel/inde
> x.html


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