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November 2010, Week 3

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Subject:
large AX3 cells
From:
Michael Myre <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Fri, 19 Nov 2010 17:04:13 -0500
Content-Type:
multipart/mixed
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text/plain (700 bytes) , Lg cells in Ax3 Phase contrast.jpg (606 kB) 
A question for the community.

As of late, our WT AX3 line contains a proportion of large cells, that
were not there in the past. They also appear when started from fresh
stocks and I'm not sure what is going on. Has anyone seen this before?
They're grown in HL5 on tissue culture dishes. It is strange as other
strains are not doing this. I've attached an example image.

Any advice would be appreciated.

Best,

Mike

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Michael A. Myre, PhD
Center for Human Genetic Research
Massachusetts General Hospital
Harvard Medical School
Richard B. Simches Research Center
185 Cambridge St., CPZN 5.612A
Boston, MA  02114
Ph. 617-643-5536
Fax 617-726-5735
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