dictyNews
Electronic Edition
Volume 47, number 8
April 2, 2021
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Abstracts
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A sub-population of Dictyostelium discoideum cells shows extremely
high sensitivity to cAMP for directional migration
Daisuke Ohtsuka, Nobutoshi Ota, Satoshi Amaya, Satomi Matsuoka,
Yo Tanaka, Masahiro Ueda
Graduate School of Science and Graduate School of Frontier
Biosciences, Osaka University, Suita, Osaka, 565-0871, Japan
Biochemical and Biophysical Research Communications,
Volume 554, 21 May 2021, Pages 131-137
The chemotaxis of Dictysotelium discoideum cells in response to a
chemical gradient of cyclic adenosine 3’,5’-monophosphate (cAMP)
was studied using a newly designed microfluidic device. The device
consists of 800 cell-sized channels in parallel, each 4 μm wide, 5 μm
high, and 100 μm long, allowing us to prepare the same chemical
gradient in all channels and observe the motility of 500-1000
individual cells simultaneously. The percentage of cells that exhibited
directed migration was determined for various cAMP concentrations
ranging from 0.1 pM to 10 μM. The results show that chemotaxis was
highest at 100 nM cAMP, consistent with previous observations. At
concentrations as low as 10 pM, about 16% of cells still exhibited
chemotaxis, suggesting that the receptor occupancy of only 6 cAMP
molecules/cell can induce chemotaxis in very sensitive cells. At
100 pM cAMP, chemotaxis was suppressed due to the self-production
and secretion of intracellular cAMP induced by extracellular cAMP.
Overall, systematic observations of a large number of individual cells
under the same chemical gradients revealed the heterogeneity of
chemotaxis responses in a genetically homogeneous cell population,
especially the existence of a sub-population with extremely high
sensitivity for chemotaxis.
submitted by: Satomi Matsuoka [[log in to unmask]]
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