dictyNews
Electronic Edition
Volume 46, number 10
April 3, 2020
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Abstracts
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A Dictyostelium discoideum mitochondrial fluorescent tagging vector
that does not affect respiratory function
Christopher J Perry 1, Eleanor C. Warren 1, Joseph L. Damstra-Oddy 1,
Claire Storey 2, Lisa M. Francione 2, Sarah J. Annesley 2, Paul R.
Fisher 2, Annette Müller-Taubenberger 3, Robin S.B. Williams 1*
1 Centre for Biomedical Sciences, School of Biological Sciences,
Royal Holloway University of London, Egham, Surrey, TW20 0EX;
2 Department of Physiology, Anatomy and Microbiology, La Trobe
University, Melbourne Victoria, 3086, Australia;
3 Department of Cell Biology, Biomedical Center, LMU Munich,
82152 Planegg-Martinsried, Germany.
Accepted in Biochemistry and Biophysics Reports
https://www.sciencedirect.com/science/article/pii/S2405580820300601
Visualizing mitochondria in living Dictyostelium discoideum cells using
fluorescent dyes is often problematic due to variability in staining,
metabolism of the dyes, and unknown potential effects of the dyes on
mitochondrial function. We show that fluorescent labelling of
mitochondria, using an N-terminal mitochondrial localization sequence
derived from the D. discoideum protein GcvH1 (glycine cleavage system
H1) attached to a red fluorescent protein enables clear mitochondrial
imaging. We also show that this labelling has no effect upon
mitochondria load or respiratory function.
submitted by: Robin Williams [[log in to unmask]]
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