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February 2009, Week 4

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Subject:
Re: hygromycin concentrations
From:
Anthony Kowal <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Fri, 27 Feb 2009 15:24:25 -0600
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text/plain
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text/plain (39 lines) 
Hi Vanessa,

In the past, we have used 36ug/ml Hygromycin for selection of
transformants.  If you are using 50ug/ul, I would think that you are
probably killing off your cells.

Tony



On Fri, Feb 27, 2009 at 1:55 PM, Vanessa Mcmains
<[log in to unmask]> wrote:
> Dicty community,
>
> I am using hygromycin as a selectable marker for overexpression of a gene. I
> do not see high expression using 50ug/ul hygromycin for selection. What is
> the highest concentration that anyone has used in Dicty to successfully
> select for overexpressing cells using hygromycin?
>
> Thanks,
> Vanessa
>



-- 
Tony Kowal
Graduate Student
Chisholm Laboratory

Northwestern University
303 E. Superior
Lurie Building
Room 7-250
Chicago, IL 60611

Lab: 312 503 4169
e-mail: [log in to unmask]

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