Dear Charlotte,

When we have problems with yeast, usually the incubation chamber/room is the
problem. We clean and desinfect then all surfaces of the incubator.
Especially if you have an incubation room with air condition (like we have)
water condensation might be a problem.

Good luck,

Sascha.


Am 01.07.2013 22:15 Uhr schrieb "charlotte Riviere" unter
<[log in to unmask]>:

> Dear all,
> 
> We had some problem in culturing DH1 strain lately in HL5 medium
> (Formedium) : we have lots of yeast appearing after few days that we can
> not get rid off.
> 
> Is there any culture tricks to avoid such yeast contamination?
> (The medium are autoclaved and filtered @0.22 µm)
> 
> Best
> 
> Charlotte
> 
> --
> Charlotte Riviere
> Institut Lumière Matière (UMR5306 UCB Lyon 1 / CNRS)
> Domaine Scientifique de la Doua
> Bâtiment Léon Brillouin
> 43 Boulevard du 11 novembre 1918
> 69622 Villeurbanne France
> Tél : +(33) (0)4 72 43 27 96 Fax : +(33) (0)4 72 43 26 48
> [log in to unmask]

-- 
Dr. Sascha Thewes
Institut für Biologie - Mikrobiologie
Fachbereich Biologie, Chemie, Pharmazie
Freie Universität Berlin
Königin-Luise-Str. 12-16
D-14195 Berlin

Tel.: 030-838-53373
Fax: 030-838-57773
Email: [log in to unmask]
Web: 
http://www.biologie.fu-berlin.de/arbeitsgruppen/mikrobiologie/ag_mutzel/inde
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