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| Date: | Fri, 6 Nov 2009 15:07:52 +0000 |
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We tried two standard inhibitors, I seem to remember MG132 and
lactacystin, without luck.
Karl Saxe found one inhibitor, I believe lactacystin, in lysates, and
found it worked. This would imply a permeability or efflux issue
rather than lack of specificity.
Robert
On 6 Nov 2009, at 14:11, Thomas Egelhoff wrote:
> Hi Dicty folks:
> We have some studies where we would like to block proteolysis in
> live Dicty cells. Has anyone tried MG132 or other proteosome
> inhibitors on Dicty? I see no pubmed refs to MG132, I am wondering
> does it not work or has no one tried it?
> Thanks,
> Tom Egelhoff
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Professor Robert Insall
CR-UK Beatson Institute for Cancer Research
Switchback Road, Bearsden
Glasgow G61 1BD, UK
Tel. (0/44) 141 330 4005
Web http://www.beatson.gla.ac.uk/robert_insall
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