dictyNews
Electronic Edition
Volume 50, number 5
April 12, 2024

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Abstracts
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The chromosomes of Dictyostelium giganteum



Rishikesh Kumar1,2*, Pooja S. Kulshreshtha1, Vidyanand 
Nanjundiah1 and Jayaram S. Kadandale1*

1 Centre for Human Genetics, Electronic City (Phase I), 
Bengaluru 560100, India; 
2 Department of Zoology, Maltidhari College, Naubatpur, Patna 
801109, India

* Correspondence:[log in to unmask], 
[log in to unmask]

Journal of Chromosomes, Accepted

Abstract: We examined the karyotype of six natural isolates of 
growing Dictyostelium giganteum cells. All six had five haploid 
chromosomes. No meiotic figures were seen. The chromosomes can 
be classified into three groups, namely group 1 with two large-
sized chromosomes, group 2 with one medium-sized chromosome and 
group 3 with two small-sized chromosomes. Fluorescence in-situ 
hybridisation was carried out using conserved D. discoideum 
centromeric DNA sequences as probes. From it, we infer that two 
chromosomes are sub-metacentric, one is metacentric and two are 
telocentric. The genome size of D.discoideum is 34.042Mb and the 
genome of an axenic strain has been completely sequenced. 
Preliminary results from work in progress indicate that the size 
of the D. giganteum nuclear genome is about 32 Mb (average of 6 
strains; unpublished data). Based on that, and from the relative 
sizes of the various chromosomes in D. giganteum, we estimate 
their genomic contents as 11.07Mb, 9.73Mb, 5.40Mb, 3.11Mb and 
2.66 Mb for chromosome Nos. 1, 2, (both sub-metacentric) 3 
(metacentric), 4, and 5 (both telocentric) respectively.


Submitted by Rishikesh Kumar [[log in to unmask]]
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