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April 2011, Week 2

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Subject:
GST-fusion protein purification from Dicty
From:
"Damer, Cynthia Kay" <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Mon, 11 Apr 2011 18:06:34 +0000
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Dear Colleagues, 

Does someone have a protocol for purifying GST-fusion proteins from
Dictyostelium cells?  I'm most concerned with finding a way to lyse the
cells.  I used to use a French Press, but no longer have access to one.

Thanks, 

Cynthia 

Cynthia K. Damer
Associate Professor
Biology Department
Brooks Hall Room 229
Central Michigan University
Mount Pleasant, MI 48859
(989) 774-3455

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