dictyNews
Electronic Edition
Volume 49, number 32
December 19, 2023
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Abstracts
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From biosynthesis and beyond – Loss or overexpression of the
cytokinin synthesis gene, iptA, alters cytokinesis and mitochondrial
and amino acid metabolism in Dictyostelium discoideum
Megan M. Aoki 1, Anna B. Kisiala 1, Sabateeshan Mathavarajah 2,
Angela Schincaglia 1, Jared Treverton 1, Elias Habib 2, Graham
Dellaire 2,3, R. J. Neil Emery 1, Craig R. Brunetti 1, and
Robert J. Huber 1
1 Department of Biology, Trent University, Peterborough, Ontario,
Canada
2 Department of Pathology, Dalhousie University, Halifax, Nova
Scotia, Canada
3 Department of Biochemistry and Molecular Biology, Dalhousie
University, Halifax, Nova Scotia, Canada
the FASEB Journal, accepted
Cytokinins (CKs) are a class of growth-promoting signaling
molecules that affect multiple cellular and developmental processes.
These phytohormones are well-studied in plants, but their presence
continues to be uncovered in organisms spanning all kingdoms,
which poses new questions about their roles and functions outside
of plant systems. Cytokinin production can be initiated by one of two
different biosynthetic enzymes, adenylate isopentenyltransfases (IPTs)
or tRNA isopentenyltransferases (tRNA-IPTs). In this study, the social
amoeba, Dictyostelium discoideum, was used to study the role of
CKs by generating deletion and overexpression strains of its single
adenylate-IPT gene, iptA. The life cycle of D. discoideum is unique
and possesses both single- and multicellular stages. Vegetative
amoebae grow and divide while food resources are plentiful, and
multicellular development is initiated upon starvation, which includes
distinct life cycle stages. CKs are produced in D. discoideum
throughout its life cycle and their functions have been well-studied
during the later stages of multicellular development of D. discoideum.
To investigate potential expanded roles of CKs, this study focused
on vegetative growth and early developmental stages. We found that
iptA-deficiency results in cytokinesis defects, and both iptA-deficiency
and overexpression results in dysregulated tricarboxylic acid (TCA)
cycle and amino acid metabolism, as well as increased levels of
adenosine monophosphate (AMP). Collectively, these findings extend
our understanding of CK function in amoebae, indicating that iptA
loss and overexpression alter biological processes during vegetative
growth that are distinct from those reported during later development.
Submitted by Robert Huber [[log in to unmask]]
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[End dictyNews, volume 49, number 32]
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