Not likely.
Then again, it depends on which tissue site we sample for and when. The most common metagenomics sampling of humans is fecal sequencing, and the databases used are pretty tuned for bacterial detection and classification. Dicty would be a vanishingly tiny component, if at all. But it also depends on the circumstance: metagenomics is often a comparative approach, where one seeks out a relative change in response to a treatment.
Now, the microbiome *of* Dicty - there’s some interesting stuff there.
R
> On Dec 15, 2021, at 10:09 AM, Alexander Sobko <[log in to unmask]> wrote:
>
> ***CAUTION:*** This email is not from a BCM Source. Only click links or open attachments you know are safe.
> Never attempted to trace Dicty development beyond 1 day (25 hours per day - no more than that!), so possibly missed those rings...
>
> Non-related curious question to experienced Dicty investigators: if we take someone who studies Dicty for several years and subject this person to metagenomics microbiome sequencing - can we ID Dicty in researcher's microbiome among other cool bacteria, fungi, viruses?
>
> Best,
>
> AS
>
> On Wed, Dec 15, 2021 at 5:05 PM Peter Devreotes <[log in to unmask]> wrote:
> Has anyone noticed that as plaques become very large, if you step back at look at it, sometimes there appear to be “rings” of more or less abundant fruiting bodies? I think these would correspond to being about one day apart. I sometimes wondered if this was a circadian but never followed up.
>
>
>
> From: DICTY <[log in to unmask]> On Behalf Of Thierry Soldati
> Sent: Tuesday, December 14, 2021 2:10 PM
> To: [log in to unmask]
> Subject: Re: [DICTY] National Geographic Dictyostelium footage
>
>
>
>
> External Email - Use Caution
>
>
>
>
>
> A colleague of mine, Ueli Schibler, one of the kings of circadian rhythms (notably in cells including in culture) used to ask whether Dicty has it … and, even though there are likely many ways to achieve a circadian clock … Dicty has none of the molecular ingredients identified in other systems, not even cryptochromes that would be necessary to sense light … But on the other hand, I had learned from the Gerisch “ritual” of keeping Dicty in constant light instead of cycling, because the switch from dark to light was thought (or shown ?) to impact some aspects of Dicty life.
>
> Any reaction or input !?
>
> Cheers,
>
>
>
> Thierry
>
>
>
>
> On 14 Dec 2021, at 18:32, Robert Kay <[log in to unmask]> wrote:
>
>
>
> We did the circadian experiments with our colleague John O'Neill, whose
> field it is. Yes, I believe the cells were light/dark entrained and then
> allowed to oscillate freely in the dark, if they would. I think we used a
> luminescent redox reporter.
>
> Just in case anybody is seriously wanting to try these experiments, I
> would say don't be too put off by our failure! There are probably lots of
> ways of making this experiment fail, and, who knows, we may have found
> one!
>
> Rob
>
>
>
> Hi all,
> Lovely videos and engaging discussion! On the circadian rhythm question,
> it
> might depend on what you measure. Having said that, I looked many, many
> years ago. measuring phototaxis and found nothing. However I never tried
> anything more nuanced, like first entraining any such rhythms with a
> day/night cycle. That should probably be done to answer the question
> properly - did you try that Rob?
> Cheers
> Paul
>
> Professor Paul R Fisher
>
> Emeritus Professor of Microbiology
>
> La Trobe University
>
> VIC 3086
>
> Australia.
>
> Tel + 61 3 9479 2229
> Email [log in to unmask]
>
>
> On Tue, 14 Dec 2021 at 10:07, Robert Kay <[log in to unmask]> wrote:
>
>
>
> Thanks Thierry and Robin - reminds us how beautiful an organism we work
> on!
>
> Have just been alerted by my brother-in-law to a recent BBC program:
> 'The
> Blob: A Genius without a Brain'.
> It's about that other slime mould and features a hilarious story line
> with
> beautiful time-lapse movies of maze solving and such, from French and
> Japanese labs.
> In the UK its on BBC iPlayer and also on YouTube:
>
> https://urldefense.com/v3/__https://www.youtube.com/watch?v=jbIiRk8s02o__;!!Dq0X2DkFhyF93HkjWTBQKhk!CS8LTeug2BEs90B-JufPJpW4WYfg0gmHjn_Pm0fHl2Ml6VhLxfCD-d8ILMJzkSOH-rDvNaIVMHhL-w$
>
> And going back, there is the classic BBC Horizon program: 'Professor
> Bonner and the Slime Moulds' from 1984. Lots of engaging John Bonner
> plus
> Dicty time lapse, including the dreaded 'Alien' sequence of
> Dictyostelium
> caveatum bursting out of a slug.....
> Used to be shown at Dicty conferences
>
> In answer to Richard's question, yes we looked for circadian rhythms in
> Dicty, and No, we didn't find any!
> Rob
>
>
>
> Dear All,
>
> Surfing on that wave, I would like to share something with you ...
> One of my colleagues in Geneva, Marko Kaksonen (CC), an expert at
> endocytosis, mainly in yeast, fell in love with Dicty and … took it
>
> home
> !
>
>
> He made some time-lapse movies of development that are possibly among
>
> the
>
>
> best ever.
> Petra Fey accepted to post it on dictybase :
>
> https://urldefense.com/v3/__http://dictybase.org/Multimedia/development/Dd*20timelapse*206*20days_Kaksonen.mp4__;JSUl!!Dq0X2DkFhyF93HkjWTBQKhk!FYOgiRD1eUsKjIHlkzRqBM6rI5NQBh9i6RPzqppjmFD5LjYMpFOIEQuH2FCfhxXIwZ90OAUCeRIa-g$
>
>
> Enjoy and share further !!
>
> Thierry
>
>
>
>
> On 10 Dec 2021, at 17:03, Williams, Robin <[log in to unmask]
> <mailto:[log in to unmask]>> wrote:
>
> Dear Dicty enthusiast
>
> I’m delighted to pass on the news that Disney Chanel, in conjunction
> with National Geographic, has just release a new documentary series,
> presented by Will Smith, called Welcome to Earth
> (
>
> https://urldefense.com/v3/__https://disneyplusoriginals.disney.com/show/welcome-to-earth__;!!Dq0X2DkFhyF93HkjWTBQKhk!FYOgiRD1eUsKjIHlkzRqBM6rI5NQBh9i6RPzqppjmFD5LjYMpFOIEQuH2FCfhxXIwZ90OAUfe8fv1g$
>
>
>
>
>
>
>
>
>
>
>
>
>
> ================================================
> Prof. Thierry Soldati
> Department of Biochemistry
> Faculty of Science
> University of Geneva
> 30 quai Ernest Ansermet, Sciences II
> CH-1211-Genève-4, Switzerland
>
> Tel: +41-22-379-6496
> email: [log in to unmask]
> https://urldefense.com/v3/__https://www.unige.ch/sciences/biochimie/labs/thierry-soldati/research/__;!!Dq0X2DkFhyF93HkjWTBQKhk!DEcwv6GARiUsyyrxB1tcIn241PLAKLwmOYC2VQbGugeaiJoMJGNafLbE_jcKxvWiK2DZP6C-tALjIg$
> @SoldatiLab
> ================================================
>
>
>
>
>
> --
> ד"ר אלכסנדר סובקו
> טלפון: 972-52-8424850
> דואר אֶלֶקטרוֹנִי: [log in to unmask]
> אתר אינטרנט:
> https://urldefense.com/v3/__https://www.linkedin.com/in/sobko-alexander__;!!Dq0X2DkFhyF93HkjWTBQKhk!DEcwv6GARiUsyyrxB1tcIn241PLAKLwmOYC2VQbGugeaiJoMJGNafLbE_jcKxvWiK2DZP6Dxi-uLcg$
>
> --
> Cheers,
> Dr. Alex Sobko, Ph.D.
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