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June 2012, Week 2

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Subject:
Dunn chamber
From:
Wanessa Cristina DE LIMA <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Wed, 13 Jun 2012 16:55:20 +0200
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Dear all,

I’ve been trying to set up a protocol for folate chemotaxis using a Dunn chamber (DCC100, Hawksley), but without success. I tried to build a gradient (in phosphate buffer) using 0.1 to 10mM folate, observing from 30 min to 6 hours. Although my cells DO move a lot (even after 6 hs in the buffer), they don’t do it in an oriented manner. Does anyone have any idea why this is not working, what I'm doing wrongly? I thought this was a pretty straightforward protocol… 

Any suggestions/tips are more than welcomed!

Cheers, Wanessa
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