But as Robert indicated, it would be nice to have a strain that is verified as NOT pneumoniae that we could get from Dictybase stock center so we don't have to worry about potential health issues when using it in classes and the lab.  Dave

On Apr 19, 2013, at 3:06 PM, Richard Sucgang PhD <[log in to unmask]<mailto:[log in to unmask]>>
 wrote:

I forgot to mention that unlike the different Dicty lineages, we are less careful about how we bank the feeder bacteria we use. For example, what one lab calls Ka may be significantly deviated from a different lab's, perhaps even exhibiting slightly different serotypes, but as long as they grow at predictable rates in the media, and Dicty will eat them, it really doesn't impact what we do.

That said, what sequences we retrieved from the contaminating bacteria matched the Kp genome well enough to identify them as such. Something to consider as a genome project is executed.

On Apr 19, 2013, at 1:22 PM, Pierre Cosson <[log in to unmask]<mailto:[log in to unmask]>> wrote:

Dear all,
Has the genome of the dicty Klebsiella (Ka) strain ever been sequenced? And does anybody have information about this bug (e.g. serotype)? If not we may be willing to give it a try.
Best
Pierre Cosson



Pierre Cosson
Centre Medical Universitaire
Dpt of Cell Physiology and Metabolism
1 rue Michel Servet
CH1211 Geneva 4, Switzerland

Email:[log in to unmask]<http://unige.ch>
Tel. (41) 22 379 5293
Fax (41) 22 379 5338

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David Knecht, Ph.D.
Professor and Head of Microscopy Facility
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)