Hi Dave-
I vaguely remember that Arturo De Lozanne was trying to express something from mice or humans in Dicty, getting very low expression, and then changed the first dozen or so codons to Dicty-favored codons and then got good expression.  

Richard Gomer

________________________________________
From: DICTY [[log in to unmask]] on behalf of Knecht, David [[log in to unmask]]
Sent: Tuesday, September 4, 2018 10:59 AM
To: [log in to unmask]
Subject: [DICTY] codon utilization

I am having a Dicty gene synthesized primarily for expression in E. coli but I might also want to express the gene in Dictyostelium.  I am trying to decide what codons to use.  Has anyone had any experience with codon utilization issues with expression of Dicty genes in E. coli or E. coli optimized genes in Dictyostelium?  Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125