It's easy.
The secret is to add silica antifoam (autoclave it mixed with water).  If
you have large volumes (eg 15 litres) you need to stir as well as bubble
to keep all the cells in suspension.  For small volumes, like the NMR tube
that Richard mentioned, the air bubbles alone will do the trick.  See:
Eu J Biochem 136, 51 (1983)
J Cell Sci 83, 165 (1986)

Best wishes,
Rob




> Hi.
>
> There is a bit of a long and uninteresting story behind this one,
> but has anyone ever bubbled their dicty cultures for agitation
> instead of shaking?  If so, were there any complications?
>
> Peace,
>
> Jon Moore
> Assistant Professor of Biology
>  & Lab Coordinator
> Pomona College
> Claremont, California 91711
>
>