I vaguely remember that it was a common E. coli cloning or expression strain, we were trying it to avoid the biosafety people getting upset about Klebsiella, but then we went back to Ka since everyone else uses Ka
Richard Gomer
________________________________________
From: DICTY [[log in to unmask]] on behalf of Joan Strassmann [[log in to unmask]]
Sent: Saturday, April 20, 2013 10:08 AM
To: [log in to unmask]
Subject: Re: [DICTY] Ka genome
It is a strain that has been used with Dicty for a very long time, so it undoubtedly has changes, but we don't know what they are. So, I can't really answer that question. As I said, it came from a source identified as KA and only sequencing let us know it is not.
Joan
On Apr 20, 2013, at 4:40 AM, salvatore bozzaro wrote:
Hi Joan,
is it a different E. coli strain than the usual B/2 or B/r that are used with Dicty?
Best,
Salvo
On Apr 19, 2013, at 11:08 PM, Joan Strassmann wrote:
Dear Everyone,
We have a strain of E. coli that Dicty just love to eat. It is originally from Richard Gomer's lab where somewhere along the line either in our lab or his, KA got replaced by E coli. The point is Dicty love to eat it. It is not classified as pathogenic. I don't know if we've sequenced it.
We are trying to switch to using it in everything. We had to become a BL2 lab instead of BL1 (USA thing) for other reasons anyway, but still prefer this.
We could send this to the stock center.
Cheers,
Joan
On Apr 19, 2013, at 3:56 PM, Knecht, David wrote:
But as Robert indicated, it would be nice to have a strain that is verified as NOT pneumoniae that we could get from Dictybase stock center so we don't have to worry about potential health issues when using it in classes and the lab. Dave
On Apr 19, 2013, at 3:06 PM, Richard Sucgang PhD <[log in to unmask]<mailto:[log in to unmask]><mailto:[log in to unmask]>>
wrote:
I forgot to mention that unlike the different Dicty lineages, we are less careful about how we bank the feeder bacteria we use. For example, what one lab calls Ka may be significantly deviated from a different lab's, perhaps even exhibiting slightly different serotypes, but as long as they grow at predictable rates in the media, and Dicty will eat them, it really doesn't impact what we do.
That said, what sequences we retrieved from the contaminating bacteria matched the Kp genome well enough to identify them as such. Something to consider as a genome project is executed.
On Apr 19, 2013, at 1:22 PM, Pierre Cosson <[log in to unmask]<mailto:[log in to unmask]><mailto:[log in to unmask]>> wrote:
Dear all,
Has the genome of the dicty Klebsiella (Ka) strain ever been sequenced? And does anybody have information about this bug (e.g. serotype)? If not we may be willing to give it a try.
Best
Pierre Cosson
Pierre Cosson
Centre Medical Universitaire
Dpt of Cell Physiology and Metabolism
1 rue Michel Servet
CH1211 Geneva 4, Switzerland
Email:[log in to unmask]<http://unige.ch<http://unige.ch/>>
Tel. (41) 22 379 5293
Fax (41) 22 379 5338
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David Knecht, Ph.D.
Professor and Head of Microscopy Facility
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)
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Joan E. Strassmann
Professor of Biology
Department of Biology
Washington University in St. Louis
One Brookings Drive
Campus Box 1137
St. Louis MO 63130
phone: (314) 935-3527 fax: (314) 935-4432
cell: (832) 978-5961
skype: strassm
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Twitter: @JoanStrassmann
Joan E. Strassmann, Professor of Biology, Department of Biology, Washington University, One Brookings Drive, Campus Box 1137, St. Louis MO 63130
phone: (314) 935-3527 fax: (314) 935-4432, cell: (832) 978-5961skype: strassm, e-mail [log in to unmask]<mailto:[log in to unmask]>
Webpage: http://strassmannandquellerlab.wordpress.com/
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Twitter: @JoanStrassmann
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