dictyNews
Electronic Edition
Volume 48, number 10
May 27, 2022

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=========
Abstracts
=========

IMfsd8 modulates growth and the early stages of multicellular 
development in Dictyostelium discoideum

Shyong Q. Yap, William D. Kim, Robert J. Huber 

Department of Biology, Trent University, Peterborough, Ontario, Canada


Frontiers in Cell and Developmental Biology, accepted

MFSD8 is a transmembrane protein that has been reported to transport 
chloride ions across the lysosomal membrane. Mutations in MFSD8 are 
associated with a subtype of Batten disease called CLN7 disease. Batten 
disease encompasses a family of 13 inherited neurodegenerative 
lysosomal storage diseases collectively referred to as the neuronal 
ceroid lipofuscinoses (NCLs). Previous work identified an ortholog of 
human MFSD8 in the social amoeba Dictyostelium discoideum (gene: 
mfsd8, protein: Mfsd8), reported its localization to endocytic compartments, 
and demonstrated its involvement in protein secretion. In this study, we 
further characterized the effects of mfsd8 loss during D. discoideum growth 
and early stages of multicellular development. During growth, mfsd8- cells 
displayed increased rates of proliferation, pinocytosis, and expansion on 
bacterial lawns. Loss of mfsd8 also increased cell size, inhibited cytokinesis, 
affected the intracellular and extracellular levels of the quorum-sensing 
protein autocrine proliferation repressor A, and altered lysosomal enzyme 
activity. During the early stages of development, loss of mfsd8 delayed 
aggregation, which we determined was at least partly due to impaired cell-
substrate adhesion, defects in protein secretion, and alterations in 
lysosomal enzyme activity. Overall, these results show that Mfsd8 plays an 
important role in modulating a variety of processes during the growth and 
early development of D. discoideum.  


Submitted by Robert Huber [[log in to unmask]]
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