DICTY Archives

April 2018, Week 2

DICTY@LISTSERV.IT.NORTHWESTERN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
Re: Expansion microscopy
From:
Ralph Gräf <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Thu, 12 Apr 2018 07:53:31 +0200
Content-Type:
text/plain
Parts/Attachments:
text/plain (26 lines) 
Hi Robert, 
yes, it works nicely. Marianne Grafe in my lab just tried it for centrosomes, microtubles and nuclear envelope. We were not very successful with GFP and DAPI, but it worked fine with Alexa dyes and Hoechst. As a crosslinker glutaraldehyde in her hands worked better than the acrylamide derivatives.
All the best, Ralph


> Am 12.04.2018 um 00:16 schrieb Robert Insall <[log in to unmask]>:
> 
> Hey, Dictyists
> 
> Has anyone used expansion microscopy on Dictyostelium or similar cells?  It sounds like just the technique to use on little cells that are hard to resolve or make into beautiful images because of light’s limitations…
> 
> Robert
> 
> 
> --------------------------------------
> Professor Robert Insall
> Cancer Research UK Beatson Institute
> Switchback Road, Bearsden
> Glasgow G61 1BD, UK
> 
> Tel.  (0/44) 141 330 4005
> Web https://urldefense.proofpoint.com/v2/url?u=http-3A__www.beatson.gla.ac.uk_robert-5Finsall&d=DwIFaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=HTiwF6UxlUXdeVtTikT74PoK5hE8bhoeVVDJNwVMJDg&s=GEOYuPtBlIsE4FNmo-1Hmxxi7mUFRngh0VY5H1xEqnQ&e=
> Orcid 0000-0003-4898-040X
> --------------------------------------
>

ATOM RSS1 RSS2