We have done the colony expansion on agar lawns, its pretty simple....

Richard Gomer

________________________________________
From: DICTY [[log in to unmask]] on behalf of Kevin Swier [[log in to unmask]]
Sent: Wednesday, March 15, 2017 3:24 PM
To: [log in to unmask]
Subject: Re: [DICTY] Bacterial streak Dicty growth assay

We were cloning some putative slow phagocytosers by diluting onto bacterial lawn...and noticed that the clearings of the mutants grew more slowly than those of wild type cells.  This observation did not repeat in subsequent assays, but that may be that our mutants were just no different than wild type cells.

Nonetheless, this seems to be another way to assay growth on bacteria as you could have many clones per plate and measure the change in diameter of the clearings over the first few days.  We have not gotten around to testing the assay on known mutants so would be happy to hear if anyone has thoughts about this or has tried this.

Kevin Swier
Professor of Biology
Chicago State University
9501 S King Drive
Chicago, IL  60628

On Tue, Mar 14, 2017 at 2:39 PM, Knecht, David <[log in to unmask]<mailto:[log in to unmask]>> wrote:
I have a vague recollection of a paper where someone used a streak assay to compare rates of consumption of different types of bacteria by Dictyosteleids.  My mental image is an agar plate with several linear streaks of bacteria.  The Dicty were inoculated at one end and the rate at which they moved up and cleared the line used as a measure of Dicty growth.  Perhaps it was a way to compare whether they consumed that type of bacteria at all.   Can anyone point me toward an assay like that?  It sounds simple, but I suspect there are tricks.  Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200<tel:860-486-2200>