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Date: | Fri, 12 May 2017 12:55:46 +0200 |
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I do not know of a prediction of miRNAs in Dicty. Fredrik Soederbom has
done sequencing of small RNAs and detected several miRNAs.
We have compared deep seq data from an rbdB KO (strongly reduced miRNA
accumulation) and agnA KO (accumulated miRNAs) and have detected some
more. There is probably more to find by careful data mining. If anybody
is interested we can probably make the deep seq data available to those
who make the best offer ;-).
The rbdB paper is here:
Meier D, Kruse J, Buttlar J, Friedrich M,
Zenk F, Boesler B, et al. (2016) Analysis of the
Microprocessor in Dictyostelium: The Role of RbdB, a
dsRNA Binding Protein. PLoS Genet 12(6):
e1006057. doi:10.1371/journal.pgen.1006057
Target prediction is difficult. Fredrik has tried and we have tried. Our
attempts for target validation were, however, not really convincing.
regards
wolfgang
Am 12.05.2017 um 11:02 schrieb mona saad:
> Hello,
> II would ask you if there is a tool to predict the microRNAs present
> in dictyostelium and their probable target
> thank you
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