Yes, we had this problem about four years ago - very disconcerting when you
study cell division.  The only comforting part was that the mutants were
more severely affected.  We ultimately decided it was the lot of proteose
peptone that we had at the time.  Currently, we have had good luck with the
media from Formedium, and I am not just saying that because Formedium is
sponsoring Dicty 2011!

Best wishes,

Doug

Douglas N. Robinson, Ph.D.
Associate Professor of Cell Biology,
Pharmacology and Molecular Sciences,
Chemical and Biomolecular Engineering
Center for Cell Dynamics and INBT
Johns Hopkins School of Medicine
725 N. Wolfe St.
Baltimore, MD 21205
ph: 410-502-2850
fax: 410-955-4129
email: [log in to unmask]
URL: http://esgweb1.nts.jhu.edu/cellbio/robinson

-----Original Message-----
From: DICTY [mailto:[log in to unmask]] On Behalf Of Michael
Myre
Sent: Friday, November 19, 2010 5:04 PM
To: [log in to unmask]
Subject: [DICTY - 452] large AX3 cells

A question for the community.

As of late, our WT AX3 line contains a proportion of large cells, that were
not there in the past. They also appear when started from fresh stocks and
I'm not sure what is going on. Has anyone seen this before?
They're grown in HL5 on tissue culture dishes. It is strange as other
strains are not doing this. I've attached an example image.

Any advice would be appreciated.

Best,

Mike

----------------------------------------
Michael A. Myre, PhD
Center for Human Genetic Research
Massachusetts General Hospital
Harvard Medical School
Richard B. Simches Research Center
185 Cambridge St., CPZN 5.612A
Boston, MA  02114
Ph. 617-643-5536
Fax 617-726-5735
[log in to unmask]
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